A new type of copolymer containing two alternating segments, poly-(styrene-alt-maleic anhydride)-co-poly (7-(4-vinylbenzyloxyl)-4-methylcoumarin-alt-maleic anhydride) P(St-alt-MAn)-co-P(VM-alt-MAn) (PSMVM), was prepared. The copolymer self-assembled into nanoparticles with internal microphase structures in water because the hydrophilicity of segment P(VM-alt-MAn) is higher than that of P(St-alt-MAn). The particle size, morphology, zeta potential and surface properties and their dependence on the pH and slat concentrations were studied with a combination of techniques. The nanoparticles of PSMVM showed surface activity and pH sensitivity for producing Pickering oil-in-water emulsions. The emulsion volume increased and the size of oil/water droplet decreased with increasing salt concentration. Furthermore, cross-linked nanoparticles (CLPs) were obtained by photo-dimerization of the pendent coumarin groups in PSMVM under UV irradiation. The emulsions produced by using the CLPs as emulsifiers showed even better stability upon standing. Solid oil-phase droplets were obtained by preparation of CLPs-stabilized Pickering emulsions with an oil phase of styrene containing the initiator AIBN followed by the polymerization of styrene. Thus, the enrichment and aggregation of the CLPs on the emulsion droplets was visible because the solid droplets remained unchanged during the SEM sample preparation. (C) 2010 Elsevier Inc. All rights reserved.