Dioscorea zingiberensis C. H. Wright (DZW) has been used as a traditional Chinese medicinal plant for the extraction of diosgenin, which is a starting material for the semisynthesis of drugs of steroidal hormones such as progesterone and testosterone. In this study, the changes in contents of three spirostanol saponins, i.e., zingiberensis newsaponin, deltonin, and prosapogenin A of dioscin, during enzymatic hydrolysis of DZW were monitored. The results show that xylanase leads to a 212% increase in the content of spirostanol saponins, as compared with that in the raw DZW rhizome, while the other three enzymes induced an similar to 22% increase. Therefore, xylanase hydrolysis of DZW would be a promising approach to enhance the content of spirostanol saponins, as well as the diosgenin yield.