The process of stripping lactic acid with different stripping agents was investigated for two organic-phase systems containing trioctylamine (TOA). On the basis of theoretical considerations for extraction mechanism, equations for stripping were developed. The theoretical values of the extent of stripping and the concentrations of lactic acid in organic and aqueous phases were compared with experimental values in the cases of water, NaOH, NaCl, HCl, and carbonates as the stripping agents. The possibilities of repeated use of the extractants in extraction/stripping runs were investigated. The best results were obtained with a system containing 20% TOA, 30% dodecane, and 50% decanol with (NR4)HCO3 as the stripping agent.