A jetting fluidized bed with acoustic assistance was employed to investigate the jet penetration depth and particle concentration profiles using an optical fiber probe. One type of FCC and two types of quartz sand particles were used as bed materials in this study. Experimental results indicated that the jet penetration depth is increased with increasing fluidizing number, jet nozzle diameter, and jet gas velocity, respectively. The jet penetration depth is decreased with an increase in particle diameter and in particle density. The semitheoretical correlations were developed to predict the jet penetration depth with and without the sound assistance. In the bubbling region, particle concentration increased with an increase in sound pressure level, which had a maximal value at a sound frequency of 150 Hz. The sound excitation had a slight influence on the particle concentration profiles in the jetting region and dense-phase particle compression region.