We outline here the fabrication of a sensitive electrochemical DNA biosensor for the detection of sequence-specific target DNA Zinc oxide nanowires (ZnONWs) were first immobilized on the surface of a glassy carbon electrode. Multi-walled carbon nanotubes (MWCNTs) with carboxyl groups were then dropped onto the surface of the ZnONWs Gold nanoparticles (AuNPs) were subsequently introduced to the surface of the MWNTs/ZnONWs by electrochemical deposition. A single-stranded DNA probe with a thiol group at the end (HS-ssDNA) was covalently immobilized on the surface of the AuNPs by forming an Au-S bond. Scanning electron microscopy (SEM) and cyclic voltammetry (CV) were used to investigate the film assembly process. Differential pulse voltammetry (DPV) was used to monitor DNA hybridization by measuring the electrochemical signals of [Ru(NH3)(6)](3+) bounding to double-stranded DNA (dsDNA). The incorporation of ZnONWs and MWCNTs in this sensor design significantly enhances the sensitivity and the selectivity This DNA biosensor can detect the target DNA quantitatively in the range of 1.0 x 10(-13) to 1 0 x 10(-7) M. with a detection limit of 3 5 x 10(-14) M (S/N = 3) In addition, the DNA biosensor exhibits excellent selectivity, even for single-mismatched DNA detection (C) 2010 Elsevier Ltd All rights reserved