Silk fibroin microspheres prepared by the water-in-oil emulsion solvent diffusion method for protein delivery

Prasong Srihanam; Yaowalak Srisuwan; Thanonchat Imsombut; Yodthong Baimark

Korean Journal of Chemical Engineering, Vol.28, No.1, 293-297, January, 2011

DOI10.1007/s11814-010-0322-4 Export Citation

Silk fibroin microspheres prepared by the water-in-oil emulsion solvent diffusion method for protein delivery

Prasong Srihanam, Yaowalak Srisuwan, Thanonchat Imsombut, and Yodthong Baimark^†

Department of Chemistry and Center of Excellence for Innovation in Chemistry,Faculty of Science, Mahasarakham University, Mahasarakham 44150, Thailand

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Silk fibroin (SF) microspheres were prepared by the simple water-in-oil emulsion solvent diffusion method without any surfactants. Aqueous SF solution and dichloromethane were used as water and oil phases, respectively. Influence of water : oil phase ratios on SF microsphere characteristics was investigated. From FTIR spectra, the resulting SF microspheres showed predominantly random coil SF conformation. SF microspheres observed from SEM images were spherical with deflated surfaces in some cases. Particle sizes of the SF microspheres were in the range 45-92 μm. Finally, bovine serum albumin (BSA) was used as a model protein for entrapment within the SF microspheres. The BSA-loaded SF microspheres were larger than unloaded SF microspheres. In vitro release tests indicate that BSA release from the SF microspheres was influenced by BSA content.

Keywords:Silk Fibroin;Microspheres;Water-in-oil Emulsion Solvent Diffusion Method;Bovine Serum Albumin;Protein Delivery

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