A series of novel alpha-peptide ribonucleic acid (alpha PRNA) oligomers, possessing alternative alpha PRNA-Gly/Lys sequences, were newly designed, synthesized, and evaluated as the second-generation PRNA. As expected, the alpha PRNA with Lys formed stable sequence-specific complex with the complementary DNA, for which both the hydrogen-bonding interactions between the complementary nucleobase pairs and the electrostatic interactions between the ammonium cation oil the lysine side chain and the DNA phosphate anion oil the backbone are jointly responsible.