A film of carboxymethyl-beta-cyclodextrin-intercalated Zn-Al layered double hydroxide (CMCD-LDH) was investigated for selective adsorption of the nucleosides adenosine (A) and guanosine (G). The effects of pH value and adsorption time on the adsorption behavior were studied. The CMCD-LDH film shows a higher selectivity for G than A under identical conditions of G and A, which results from the ability of selective recognition of the interlayer CMCD. The kinetic studies show that adsorption of A and G by the CMCD-LDH film can be described satisfactorily by the slab diffusion model. Both the values of diffusion coefficient (D) and adsorption capability (q(e)) of G by the CMCD-LDH film are larger than those of A, demonstrating the selective adsorption of the CMCD-LDH film for nucleosides. Due to its easy preparation and manipulation, this film is expected to be successfully applied in the field of selective adsorption and separation.