In this study, the lipase catalysed esterification reaction for biodiesel production was investigated in the presence of the ionic liquid [BMIM][PF6] Unlike regular organic solvents, many ionic liquids have no vapour pressure, and are therefore considered non-volatile When used in systems with enzyme catalysts, ionic liquids may enhance their activity, selectivity, and stability The use of an enzyme (lipase) as a catalyst, and the ionic liquid as a solvent/immobilization agent also represents an environmentally friendly, "green" technology Methyl acetate was used as the acyl acceptor as opposed to the more commonly used methanol due to the negative effects methanol and the glycerol by-product has on lipase enzyme activity The results of this research indicate that methyl oleate e, biodiesel) was successfully produced, with an 80% overall biodiesel yield in the presence of ionic liquid, at a 1 1 ratio (v/v) to the amount of oil This verified that the presence of an ionic liquid, at a specified amount, improved the activity of the lipase and the overall biodiesel yield Results also indicate the addition of ionic liquid facilitated the separation of the methyl esters from the triacetylglycerol by-product The best conditions investigated was found to be. 14 1 molar ratio between oil and acyl acceptor, 20% (w immobilised lipase/w of oil, and a temperature in the range of 48-55 degrees C However, additional purification is required in order for the produced biodiesel to meet ASTM standards