Biotechnology Letters, Vol.32, No.10, 1481-1486, 2010
Reconstruction of the UDP-N-acetylglucosamine biosynthetic pathway in cell-free system
A functional glucokinase (YqgR), N-acetylglucosamine-phosphate mutase (Agm1) and N-acetylglucosamine-1-phosphate uridyltransferase (GlmU) were synthesized simultaneously in an Escherichia coli cell-free system. These three enzymes were coupled to reconstruct a biosynthetic pathway which could produce UDP-N-acetylglucosamine with N-acetylglucosamine as the substrate.
Keywords:Biosynthetic pathway;Cell-free protein synthesis;Glucokinase;N-acetylglucosamine-phosphate mutase;N-acetylglucosamine-1-phosphate uridyltransferase;Uridine 5 '-diphospho N-acetylglucosamine