Bioleaching of spent catalyst were carried out using Acidithiobacillus type of microorganisms. Various leaching parameters like contact time, Fe(II) concentration, particle size, pulp densities, pH and temperature were studied in details. All the four metal ions like Ni, V, Mo and Al followed dual kinetics, i.e., initial faster followed by slower rate. The leaching kinetics of Ni and V observed to be higher compared to that of Mo and Al. The thermodynamic parameters like ΔG, ΔH and ΔS for all metals were calculated. The leaching kinetics followed first order rate. Rates of dissolution of Al, V and Ni increased, and Mo decreased with increase of Fe(II) addition whereas that of all metals decreased with increase of pulp density and particle size. Leaching kinetics of Al, Mo, V increased with decrease of pH. Variation of initial pH of the leaching medium showed an inadequate effect on Ni dissolution. The rate determining step found to be pore diffusion controlled. The correlation between observed and theoretical values of leaching efficiency for different parameters was evaluated using Multi-Linear Regression Analyses which showed the significance of the leaching. A total of 5 factors were evaluated by data reduction technique using Principal Component Analysis.