Tensile properties, cell adhesion, and drug release behavior of chitosan-silver-gelatin nanohybrid films and scaffolds

V. K. Rana; Omkar S. Kushwaha; RajPal Singh; Satyendra Mishra; Chang-Sik Ha

Macromolecular Research, Vol.18, No.9, 845-852, September, 2010

DOI10.1007/s13233-010-0905-2 Export Citation

Tensile properties, cell adhesion, and drug release behavior of chitosan-silver-gelatin nanohybrid films and scaffolds

V. K. Rana, Omkar S. Kushwaha, RajPal Singh^†, Satyendra Mishra¹, and Chang-Sik Ha²

Division of Polymer Science and Engineering, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411 008, India
¹Department of Chemical Technology, North Maharashtra University, Jalgaon, 425001, India
²Department of Polymer Science and Engineering, Pusan National University, Busan, 609-735, Korea

E-mail:

Drug-loaded nanohybrid films and porous scaffolds were prepared using chitosan, Ag nanoparticles and gelatin using a solution casting and freeze-drying method, respectively. Gelatin was used to incorporate the cell onto the surface of the scaffolds and the nanohybrid films. 1-[2-hydroxyethyl]-2-methyl-5-nitroimidazole) (Metronidazole (MTZ) was used as a model drug. The small percentage of Ag nanoparticles in the nanohybrid films and scaffolds produced significantly higher cell proliferation and levels of drug release. The tensile properties showed improvement in strength by Ag nanoparticles reinforcement at the expense of elongation.

Keywords:chitosan-Ag-gelatin nanohybrids;drug release;carcinogenic metronidazole;nano-hybrids;scaffolds;cell proliferation

[References]

Thapa PB, Whitlock JA, Worrell KGB, Gideon P, Mitchel EF, Roberson P, Pais R, Ray WA, Cancer, 83, 1461 (1998)
World Health Organization, WHO Drug Information, 13, 249 (1999).
Carey JC, Klebanoff MA, Am. J. Obstet. Gynecol., 192, 1341 (2005)
Kumar MNVR, Muzzarelli RAA, Muzzarelli C, Sashiwa H, Domb AJ, Chem. Rev., 104(12), 6017 (2004)
Jeong YI, Seo DH, Kim DG, Choi C, Jang MK, Nah JW, Park Y, Macromol. Res., 17(7), 538 (2009)
Diorio JP, Stojanovic L, Yardimci A, Amrani DL, Helgerson S, Vega F, Proc. Microscopy and Microanalysis, Honolulu, Hawaii, USA, July 31.August 4, 2005.
Ito A, Mase A, Takizawa Y, Shinkai M, Honda H, Hata K, Ueda M, Kobayashi T, J. Biosci. Bioeng., 95(2), 196 (2003)
Jeong SI, Lee YM, Shin H, Macromol. Res., 16(6), 567 (2008)
Mizuno A, Mitsuiki M, Motoki M, J. Agric. Food Chem., 48, 3286 (2000)
Min KD, Park WH, Youk JH, Kwark YJ, Macromol. Res., 16(7), 626 (2008)
Grier N, Silver and Its Compound, 3rd edition, Block SS, Ed., Lea & Febiger, Philadelphia, 1983, vol. 375.
Peppas NA, Lustig SR, Ann. N.Y. Acad. Sci., 446, 26 (1985)
Do JH, An JH, Joun YS, Chung DJ, Kim JH, Macromol. Res., 16(8), 695 (2008)
Zhou YS, Yang DZ, Chen XM, Xu Q, Lu FM, Nie J, Biomacromolecules, 9(1), 349 (2008)
Pattani A, Patravale VB, Panicker L, Potdar PD, Mol. Pharm., 6, 345 (2009)
Guo R, Zhang LY, Qian HQ, Li RT, Jiang XQ, Liu BR, Langmuir, 26(8), 5428 (2010)
Giannelis EP, Adv. Mater., 8(1), 29 (1996)
Agnihotrym SA, Mallikarjuna NN, Aminabhavi TM, J. Control. Release, 100, 5 (2004)
Liu BS, Huang TB, Macromol. Biosci., 8, 932 (2008)
Hong RL, Yu JY, J. Biomed. Mater., 71, 52 (2004)

[Referenced By]

[Related Articles]

[1] Thapa PB, Whitlock JA, Worrell KGB, Gideon P, Mitchel EF, Roberson P, Pais R, Ray WA, Cancer, 83, 1461 (1998)

[2] World Health Organization, WHO Drug Information, 13, 249 (1999).

[3] Carey JC, Klebanoff MA, Am. J. Obstet. Gynecol., 192, 1341 (2005)

[4] Kumar MNVR, Muzzarelli RAA, Muzzarelli C, Sashiwa H, Domb AJ, Chem. Rev., 104(12), 6017 (2004)

[5] Jeong YI, Seo DH, Kim DG, Choi C, Jang MK, Nah JW, Park Y, Macromol. Res., 17(7), 538 (2009)

[6] Diorio JP, Stojanovic L, Yardimci A, Amrani DL, Helgerson S, Vega F, Proc. Microscopy and Microanalysis, Honolulu, Hawaii, USA, July 31.August 4, 2005.

[7] Ito A, Mase A, Takizawa Y, Shinkai M, Honda H, Hata K, Ueda M, Kobayashi T, J. Biosci. Bioeng., 95(2), 196 (2003)

[8] Jeong SI, Lee YM, Shin H, Macromol. Res., 16(6), 567 (2008)

[9] Mizuno A, Mitsuiki M, Motoki M, J. Agric. Food Chem., 48, 3286 (2000)

[10] Min KD, Park WH, Youk JH, Kwark YJ, Macromol. Res., 16(7), 626 (2008)

[11] Grier N, Silver and Its Compound, 3rd edition, Block SS, Ed., Lea & Febiger, Philadelphia, 1983, vol. 375.

[12] Peppas NA, Lustig SR, Ann. N.Y. Acad. Sci., 446, 26 (1985)

[13] Do JH, An JH, Joun YS, Chung DJ, Kim JH, Macromol. Res., 16(8), 695 (2008)

[14] Zhou YS, Yang DZ, Chen XM, Xu Q, Lu FM, Nie J, Biomacromolecules, 9(1), 349 (2008)

[15] Pattani A, Patravale VB, Panicker L, Potdar PD, Mol. Pharm., 6, 345 (2009)

[16] Guo R, Zhang LY, Qian HQ, Li RT, Jiang XQ, Liu BR, Langmuir, 26(8), 5428 (2010)

[17] Giannelis EP, Adv. Mater., 8(1), 29 (1996)

[18] Agnihotrym SA, Mallikarjuna NN, Aminabhavi TM, J. Control. Release, 100, 5 (2004)

[19] Liu BS, Huang TB, Macromol. Biosci., 8, 932 (2008)

[20] Hong RL, Yu JY, J. Biomed. Mater., 71, 52 (2004)