We have recently reported that an alternative splicing variant of liver X receptor (LXR)-beta acts as an RNA co-activator, which is referred to as LXRBSV The in vivo role of LXRBSV is yet to be clarified The LXRBSV gene is expressed in various tissues including the liver and brain We evaluated the gene expression of LXRBSV in various regions of the brain using real-time quantitative PR assays in the current study and found that LXRBSV is abundantly expressed in the pituitary 5'-rapid amplification of cDNA ends (5'-RACE) revealed that the transcriptional start site (TSS) of LXRBSV is located 40 base pairs (bp) downstream of LXR-beta We prepared two promoter constructs -1598/+35 bp and -15981+75 bp in pGL4 for LXR-beta and LXRBSV, respectively The latter promoter construct demonstrated significantly higher activity than the former construct in GH3 cells derived from the rat pituitary On the contrary, the promoter activities of these two constructs were indistinguishable in Hepa 1-6 cells derived from mouse hepatocytes Furthermore, the promoter region specific for LXRBSV itself exerted promoter activity in GH3 cells but not in Hepa 1-6 cells Taken together, we have concluded that LXRBSV is preferentially transcribed and expressed in the pituitary, indicating that LXRBSV plays a role in regulating pituitary gene expression These data provide clues to elucidating the physiological relevance of LXRBSV (C) 2010 Elsevier Inc All rights reserved