MicroRNAs (miRNAs) comprise a post-transcriptional layer of gene regulation shown to be involved ill diverse physiological processes We aimed to Study whether regulatory networks that determine susceptibility to hypertension may involve a miRNA component Screening of loci, involved in renal water-salt balance regulation. highlighted the mineralocorticoid receptor gene NR3C2 as a potential target for several miRNAs A luciferase assay demonstrated that miR-124 and miR-135a suppress NR3C2 3'UTR reporter Construct activity 1.5- and 2.2-fold, respectively As the tested miRNAs (lid not reduce the levels of target mRNA, we suggest that the binding of miR-124 and miR-135a to NR3C2 3'UTR contributes to the translational, not transcriptional regulation of the gene Co-expression of two different miRNAs did not increase the repression of the reporter gene, indicating no additive or synergistic effects between the tested miRNAs Our results demonstrate that by repressing the mineralocorticoid receptor gene NR3C2, miR-124 and miR-135a Could participate in the regulation of ienin-angiotensin-aldosterone system and thereby might be involved in blood pressure regulation. (C) 2009 Elsevier Inc All rights reserved