A study has been made on carrier-mediated transport of uranium(VI) using a bulk liquid membrane prepared by dissolving benzoyltrifluoroacetone (HBTA) in carbon tetrachloride. The source phase comprised of a solution of UO22+ or its binary mixture with other cations such as Th4+, Hf4+, Zr4+, Fe3+, La3+, Cu2+, Co2+, Mn2+, Ni2+, and Zn2+ in aqueous solutions of pH 6.0, while 0.1 M hydrochloric acid was serving as a stripping agent in the receiving compartment. The interference from Th4+ and a few other cations could be eliminated by using trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid (DCTA) as a proper masking agent in the feed solution. Various factors influencing the transport process have been studied and an uphill transport (> 99%) of uranium(VI) from the source phase could be accomplished under optimum conditions.