The effect of temperature and solvent on the extraction of Artemisia asiatica using pressurized liquid extraction was examined. These two parameters were operated according to central composite design followed by response surface analysis to obtain the highest extraction efficiency. Determination of eupatilin was carried out by high performance liquid chromatography coupled with a diode array detector. While the rise in temperature could increase the yield of the total extract, the content of eupatilin was decreased in high temperature. In addition, high ethanol proportion maximized the contents of eupatilin in the total extract.