The influences of extraction solvents and techniques on the yield of alkaloids from the embryo of the seed of Nelumbo nucifera GAERTN, were comprehensively investigated in this work. After the preliminary tests (extraction solvents and extraction methods), several parameters, such as ethanol concentration, extraction time, the ratio of liquid to solid were optimized using an experimental design, response surface methodology, and accelerated random search algorithm (ARSA). The results showed the best experimental conditions for total alkaloids (T.A). Using ultrasound-assisted extraction were ethanol concentration: 75%, extraction time: 20min, and the ratio of liquid to solid: 30:1.