Silk-elastinlike protein polymer, SELP-815K, with eight silk and fifteen elastin units and a lysine (K) modified elastin, was genetically engineered with longer silk and elastin units compared to existing hydrogel forming analogs (SELP-415K and SELP-47K). Hydrogels of the three SELPs (with similar MWs) were investigated for their structure-function relationships. Results indicate that equilibrium swelling ratio in these hydrogels is a function of polymer structure, concentration, cure time and ionic strength of media. Swelling was not influenced by the changes in pH. Storage moduli observed by dynamic mechanical analysis and the Debye-Bueche correlation length obtained from small-angle neutron scattering provided structural insight that suggests the cross-linking densities in these hydrogels follow the order SELP-47K > SELP-815K > SELP-415K. These results allude to the importance of the length of elastin blocks in governing the spacing of the cross-linked hydrogel network and that of silk in governing the stiffness of their 3-dimensional structures. (C) 2008 Elsevier Ltd. All rights reserved.