A new method for creating Substrates made out of ordered collagen fibers, on which cells in culture can align, is proposed. The substrates call be used for research ill Cell Culture, and this research presents,I significant advance in the technology to coal implants in order to improve cell adhesion. In the procedure presented here, a molecular solution of Collagen is spread at the interface of a saline solution and air to induce fiber formation, compressed at a high speed to induce orientation and deposited on solid substrates via Langmuir-Blodgett transfer. Several interfacial techniques are employed to investigate the behavior of collagen, which is shown to be dependent oil the salt concentration of the subphase as well as the temperature, After Langmuir-Blodgett transfer, primary human fibroblasts and adipose-derived stern cells are cultured on the collagen substrates. Both types of cells respond favorably to the collagen orientation and align with the deposited fibers. The technique presented here provides a simple method to produce well-controlled, oriented collagen substrates that can be used in tissue culture research or scaffolding applications without the use of additives and/or bioincompatible materials.