화학공학소재연구정보센터
Langmuir, Vol.24, No.9, 4968-4974, 2008
Lipid bilayer elasticity measurements in giant liposomes in contact with a solubilizing surfactant
A new method to probe the modification of the elasticity of phospholipid bilayers is presented. The purpose here concerns the action of a solubilizing surfactant on a vesicle bilayer. This method is based on the measure of the under-field elongation of giant magnetic-fluid-loaded liposomes. The addition of the nonionic surfactant octyl-beta-D-glucopyranoside (OG) to vesicles at sublytic levels increases the elasticity of the membrane, as shown by the value of the bending modulus K-b, which decreases. Kb measured around 20 U for a pure 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayer indeed reaches a few U in the case of the mixed OG-DOPC bilayer. The purpose and interest of this study are to allow the determination of the membrane bending modulus before and after the addition of OG on the same magnetic liposome. Moreover, the experimental conditions used in this work allow the control of lipid and surfactant molar fractions in the mixed aggregates. Then, optical microscopy observation can be performed on samples in well-defined regions of the OG-phospholipid state diagram.