Journal of the American Chemical Society, Vol.131, No.9, 3225-3229, 2009
Biosynthesis of the Thiamin Thiazole in Bacillus subtilis: Identification of the Product of the Thiazole Synthase-Catalyzed Reaction
In this paper, we describe an optimized reconstitution of the thiamin thiazole synthase (ThiG) catalyzed reaction and demonstrate that the enzymatic product is an unanticipated dearomatized thiazole tautomer.