A microfluidic glucose/O-2 biofuel cell, delivering electrical power, is developed based on both laminar flow and biological enzyme strategies. The device consists of a Y-shaped microfluidic channel in which fuel and oxidant streams flow laminarly in parallel at gold electrode surfaces without convective mixing. At the anode, the glucose is oxidized by the enzyme glucose oxidase whereas at the cathode, the oxygen is reduced by the enzyme laccase, in the presence of specific redox mediators. Such cell design protects the anode from interfering parasite reaction Of O-2 at the anode and works with different streams of oxidant and fuel for optimal operation of the enzymes. The dependence of the flow rate on the current is evaluated in order to determine the optimum flow that would provide little to no mixing while yielding high current densities. The maximum power density delivered by the assembled biofuel cell reaches 110 mu W cm(-2) at 0.3 V with 10 mM glucose at 23 degrees C. This research demonstrates the feasibility of advanced microfabrication techniques to build an efficient microfluidic glucose/O-2 biofuel cell device. (C) 2009 Elsevier B.V. All rights reserved.