Upon one-electron oxidation, all molecules including DNA bases become more acidic in nature. For the GC base pair, experiments suggest that a facile proton transfer takes place in the G(center dot+)-C base pair from N-1 of G(center dot+) to N-3 of cytosine. This intrabase pair proton-transfer reaction has been extensively considered using theoretical methods for the gas phase, and it is predicted that the proton transfer is slightly unfavorable, in disagreement with experiment. In the present study, we consider the effect of the first hydration layer on the proton-transfer reaction in G(center dot+)-C by the use of density functional theory (DFT) using B3LYP/6-31+G** calculations of the G(center dot+)-C base pair in the presence of 6 and 11 water molecules. Under the influence of hydration of 11 waters, a facile proton transfer from N-1 of G(center dot+) to N-3 of C is predicted. The zero-point energy (ZPE)-corrected forward and backward energy barriers, for the proton transfer from N-1 of G(center dot+) to N-3 of C, was found to be 1.4 and 2.6 kcal/mol, respectively. The proton-transferred G(center dot)-(H+)C + 11H(2)O was found to be 1.2 kcal/mol more stable than G(center dot+)-C + 11H(2)O, in agreement with experiment. The present calculation demonstrates that the inclusion of the first hydration shell around the G(center dot+)-C base pair has an important effect on the internal proton-transfer energetics.