We investigated the rotational spectra of six isotopologues of pyridine-Ne-2 formed by combinations of two isotopes of the nitrogen atom (N-14 and N-15) in pyridine with two isotopes of the rare gas atoms (Ne-20 and Ne-22), by using pulsed jet Fourier transform microwave spectroscopy. We detected the C-2v symmetry conformer, denoted as [1,1], where the Ne atoms are located one on each side of the ring plane. The [2,0] species, with the two Ne atoms on the same side of the ring, was not observed. Two structural parameters, R and theta, that localize the rare gas atoms with respect to pyridine have been determined. The N-14 nuclear quadrupole coupling constants have been obtained for the isotopologues containing this nucleus.