A molecular imprinting polymer targeted to alpha-tocopherol (alpha-Toc) was developed by polymer membrane scaffold method containing calix[4]resorcarenes and showing multiple non-covalent interactions with the template. A host-guest complex was fixed in a scaffold polymer membrane consisting of polysulfone. Phase inversion of the polymer solution containing the host-guest complex was carried out from a NMP solution into water. After the membrane was formed, removal of the template was performed by treating with MeOH/AcOH solution. It was observed that the amount of alpha-Toc bound to the imprinted and non-imprinted membranes in methanol/water (2: 1, v/v%) was 41.1 +/- 0.9 and 22.2 +/- 1.1 mu mol/g-membrane, respectively. Relative to the alpha-Toc analogues, 2-napthol (2-Nap), permselective experiments were achieved with a separation factor of 14.5 for alpha-Toc retained. (C) 2009 Elsevier B.V. All rights reserved.