The different isomers of technical-grade hexachlorocyclohexane (t-HCH) including the insecticidal gamma-isomer, commonly known as lindane, have been reported to be toxic. carcinogenic and endocrine disrupters. The spatial arrangements of the chlorine atoms on different isomers and low aqueous phase solubility contribute to their persistence in environment, beta-HCH being the most resistance to transformation. The biosurfactant preparation of Peudomonas aeruginosa isolate WH-2 was evaluated for its ability to improve the aqueous phase partitioning of different isomers of HCH-muck. Further, the ability of biosurfactant preparation to emulsify HCH and n-hexadecane was checked under different conditions, usually characteristic of sites contaminated with pollutants viz. wide range of pH, temperature, and salinity. The data obtained from this study will be helpful in designing suitable bioremediation strategies for huge stock piles of HCH-muck and sites polluted by reckless use/disposal of HCH-isomers. (C) 2009 Elsevier B.V. All rights reserved.