Bagasse is a by-product of sugar milling and important fuel resource for that industry. It is a fibrous, low density material with a very wide range of particle sizes and high moisture content. The goal of this study is the development of a system based on the use of the ligninolytic enzyme manganese peroxidase (MnP) produced by Phanerochaete chrysosporium for the degradation of polycyclic aromatic hydrocarbons (PAHs). of which anthracene was selected as an example. The white rot fungus P. chrysosporium immobilized on bagasse was grown in both stationary and agitated cultures (rotary shaker, 80 rpm) using nitrogen limited growth medium to study the ability of the fungus to degrade anthracene in aqueous media. Production of MnP occurred simultaneously in nitrogen limited culture medium with the added MnSO4 at 40 ppm. The MnP activity was at relatively high level (76U1(-1)) and in this condition, the residual anthracene concentration was 16%. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.