This short Communication reports the observation that in contrast to most previously reported procedures, it is possible to prepare 3D photonic crystal structures from silica particles that have not been deliberately treated with surfactant molecules, using the Langmuir-Blodgett method. We find that colloidal particles prepared simply via the Stober method with diameters in the range 180-360 nm and dispersed in ethanol, may be effectively floated at the air/water interface and compressed into close packed layers prior to depositing the layers on a substrate. We also find, by comparing structures made with both particles treated with the surfactants 3-(trimethoxysilyl) propyl methacrylate or (3-aminopropyl)triethoxysilane and particles which have not been treated with any surfactant species, that the position of the Bragg peak and the reflectivity of the sample does not appear to be influenced by the presence of the surfactant molecules. (C) 2009 Elsevier Inc. All rights reserved.