Journal of Applied Microbiology, Vol.107, No.6, 1847-1856, 2009
Sporulation, storage and infectivity of obligate aphid pathogen Pandora nouryi grown on novel granules of broomcorn millet and polymer gel
Aims: Producing granular cultures of obligate aphid pathogen Pandora nouryi for improved sporulation and storage. Methods and Results: Small millet-gel granules were made of the mixtures of 80-95% millet powder with 5-20% polymer gel (polyacrylamide, polyacrylate or acrylate-acrylamide copolymer) and inoculated with mycelia at 30 mg biomass g-1 dry granules plus 87 center dot 5% water, followed by static incubation at 20 degrees C for 4-12 days. The fungus grew well on 12 preparations but best on that including 10% copolymer. An 8-day culture of this preparation discharged maximally 58 center dot 5 x 104 conidia mg-1 granule at 100% RH and was capable of ejecting conidia at the nonsaturated regimes of 86-97% RH. During storage at 6 degrees C, granular cultures with > 85% water content had twofold longevity (120 days) and half-decline period (34-36 days) of those stored at room temperature. The steadily high water content preserved the cultures better than that decreasing at 6 degrees C. However, conidia from 70-day-stored granules were less infective to Myzus persicae nymphs than those from fresh ones based on their LC(50)s. Conclusions: The millet-gel granules had higher sporulation capacity than reported Pandora cultures and a capability of spore discharge at nonsaturated humidity. Significance and Impact of the Study: The granular cultures are more useful for aphid control.
Keywords:aphid infectivity;Entomophthorales;Myzus persicae;Pandora nouryi;polymer millet-gel culture;sporulation capacity;storage longevity