The kinetics of oxidation of ethanol-gasoline surrogate mixtures (85-15 vol %) was studied using a fused silica jet-stirred reactor. One representative of each class constituting E85 gasoline was selected. These constituents were iso-octane, toluene, 1-hexene, and ethanol. The experiments were performed in the temperature range of 770-1220 K, at 10 atm, at four equivalence ratios (0.3, 0.6, 1, and 2), and with an initial fuel concentration of 0.2 mol %. A detailed kinetic scheme resulting from the merging of validated kinetic schemes for the oxidation of the components of the present E85 surrogate (gasoline surrogate and ethanol) was used. Good agreement between the experimental results and the computations was observed under the present JSR conditions.