Amorphous silica gel modified carbon paste electrode (CPE) offers substantial improvements in voltammetric sensitivity and selectivity towards determination of dopamine (DA). cyclic voltammetry of Fe(CN)(6)(3-/4-) as a negatively charged probe revealed that the surface of the silica gel modified carbon paste electrode had a high density of negative charge at pH 8.0. Therefore, the modified electrode adsorbed DA (pK(a) = 8.9) and enhanced its voltammetric response while repulsed ascorbic acid (AA) (pK(a) = 4.2) and uric acid (UA) (pK(a) = 5.4) and inhibited their interfering effects. The influence of various experimental parameters including percent of silica gel in the CPE, pH of solution, and accumulation time and potentials, on the voltammetric response of DA was investigated. At the optimum conditions, the analytical curve was linear for dopamine concentrations from 2.0 x 10(-7) to 1.0 x 10(-6) mol L-1 and 2.0 x 10(-6) to 1.5 x 10(-4) mol L-1 with a detection limit (3 sigma) of 4.8 x 10(-8) mol L-1. The prepared electrode was used for determination of DA spiked into DA injection and human serum samples, and very good recovery results were obtained over a wide concentration range of DA. (C) 2009 Elsevier Ltd. All rights reserved.