The nanophotocatalytic process using semiconductors with a nanostructure is one of the technologies used for the destructive oxidation of organic compounds Such as dyes. The photocatalytic oxidation of Reactive Orange 16 aqueous solution applied in textile industries was assessed by UV ray irradiation in the presence of TiO2 nanoparticles. The effect of dye initial concentration, pH, TiO2 loading and the effect of anion presence on dye degradation were investigated and the optimized conditions for maximum amount of degradation were determined. Analysis of the kinetic showed that the amount of dye photocatalytic degradation can be fitted with pseudo-first-order model. The mineralization of RO 16 dye was reported by measuring the initial and final chemical oxigen demand of the solution that was irradiated under optimized conditions.