This paper describes a glass substrate having a photocleavable poly(ethylene glycol) (PEG) designed for light-induced cell micropatterning. The Substrate changed from non-cell-adhesive to cell-adhesive by the photocleavage of PEG. Cellular patterns maintained for more than 17 days, and they were able to be changed to control cell migration and proliferation at multi- and single-cell levels by irradiating their adjacent regions during cell cultivation.