This study evaluates the applicability of the liquid handling workstation Tecan Evo Freedom 200 and 200 mu L Media Scout RoboColumns to dynamic chromatographic operations such as frontal analysis (breakthrough) and elution experiments in a high throughput screening mode. Breakthrough experiments were conducted using BSA as a model protein and the stationary phases Poros 50 D, Q Ceramic HyperD and DEAE Sepharose FE The obtained dynamic capacities at 10 and 50 IN breakthrough matched well with reference data. Elution experiments were performed applying three protein mixtures: a) lipolase and BSA, b) human growth hormone and a process-related impurity, and c) an insulin analogue and a process-related impurity. The resins used resembled a variety of different ion exchange resins. In all cases, the resulting elution curves matched well with reference measurements performed on an AKTA explorer system at 1 mL or 2 mL scale.