Effects of calcination temperature for Pt-SO42-/ZrO2-Al2O3 (PSZA) catalysts in n-hexane hydroisomerization were investigated by N-2-adsorption, XRD, TG-DTA, FTIR, XPS and H-2-TPR. An optimum calcination temperature is helpful to complete the crystallization process, resulting in better distribution of alumina into zirconia crystal and producing new acid centers responsible for higher catalytic activity.