Biotechnology Letters, Vol.30, No.10, 1789-1793, 2008
L-ribulose production from L-arabinose by an L-arabinose isomerase mutant from Geobacillus thermodenitrificans
Geobacillus thermodenitrificans, with a double-site mutation in L-arabinose isomerase, produced 95 g L-ribulose l(-1) supercript stop from 500 g L-arabinose l(-1) under optimum conditions of pH 8, 70 degrees C, and 10 units enzyme ml(-1) with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75 degrees C were 35 and 4.5 h, respectively.