Endothelial cell layer regulates several crucial physiological processes of the vascular system. The mechanism of the response of this cell layer to the flow of surrounding fluid is still largely unclear. In the present article, a comparison of the available experimental results for the intracellular calcium ion concentration and theoretical results for the extra-cellular ATP concentration obtained using a convective-diffusive mass transfer model, has been made which supports the mass transfer model for the endothelial cell response to the fluid flow. The experimental results are in excellent agreement with the calculated values assuming the effect of ATP concentration alone.