Optimization of medium for phenylalanine ammonia lyase production in Escherichia coli using response surface methodology

Jian-Dong Cui

Korean Journal of Chemical Engineering, Vol.27, No.1, 174-178, January, 2010

DOI10.1007/s11814-009-0234-3 Export Citation

Optimization of medium for phenylalanine ammonia lyase production in Escherichia coli using response surface methodology

Jian-Dong Cui^†

Hebei Fermentation Engineering Research Center, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang 050018, China

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A culture medium for phenylalanine ammonia lyase (PAL) production in E. coli was developed following preliminary studies by means of response surface methodology (RSM). The medium components having significant effect on the production were first identified using a fractional factorial design. Then, central composite design (CCD) was utilized to optimize the medium constituents and explain the combined effects of four medium constiuents, viz., glucose, yeast extract, (NH4)2HPO4 and MgSO4. A quadratic model was found to fit the PAL production. CCD revealed that the optimum values of the test variables for PAL production were glucose 28.2 g/L, yeast extract 5.01 g/L, (NH4)2HPO4 7.02 g/L and MgSO4 1.5 g/L. PAL production of 62.85 U/g, which was in agreement with the prediction, was observed in verification experiment. In comparision to the production of basal medium, 1.8-fold increase was obtained.

Keywords:Enzyme Activity;Phenylalanine Ammonia Lyase;Response Surface Method;Fermantation;Medium;Batch Culture

[References]

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Fiske MJ, Kane JF, J. Bacteriol., 160, 676 (1984)
Cui JD, Li Y, Korean J. Chem. Eng., In press (2009)
Rosler J, Krekel F, Amrhein N, Plant Physiol., 113, 175 (1997)
Orum H, Rasmussen OF, Appl. Microbiol. Biotechnol., 36, 745 (1992)
Faulkner JDB, Anson JG, Tuite MF, Gene., 143, 13 (1994)
Jia SR, Cui JD, Li Y, Biochem. Eng. J., 42, 193 (2008)
Abdel-Fattah YR, Saeed HM, Gohar YM, Process Biochem., 40, 1707 (2005)
Montgomery DC, Design and analysis of experiments, Singapore, Wiley Press, 125 (1984)
Liyana-Pathirana C, Shahidi F, Food Chem., 9, 347 (2005)
Lee H, Song M, Wang SH, Process Biochem., 38, 1685 (2003)
Bocchini DA, Alves-Prado HF, Baida LC, Process Biochem., 38, 727 (2002)
Majumader A, Goyal A, Bioresour. Technol., 99(9), 3685 (2008)
Delisa MP, Rao G, Weigand WA, Biotech. Bioeng., 6, 554 (1999)
LEE JH, YOO YJ, PAR KH, Korean J. Chem. Eng., 8(1), 39 (1991)
Haghighi B, Shahidi D, Papari MM, Najafi M, Korean J. Chem. Eng., 24(1), 1 (2007)
Sen R, J. Chem. Technol. Biotechnol., 68(3), 263 (1997)
Akhnazarova S, Kefarov V, Experiment optimization in chemistry and chemical engineering, Moscow, Mir Publishers, 135 (1982)
Khuri AI, Cornell JA, Response surfaces: design and analysis, New York, Marcel Dekker, 211 (1987)

[Referenced By]

[1] Yue HY, Yuan QP, Wang WC, Biochem. Eng. J., 37, 231 (2007)

[2] Fiske MJ, Kane JF, J. Bacteriol., 160, 676 (1984)

[3] Cui JD, Li Y, Korean J. Chem. Eng., In press (2009)

[4] Rosler J, Krekel F, Amrhein N, Plant Physiol., 113, 175 (1997)

[5] Orum H, Rasmussen OF, Appl. Microbiol. Biotechnol., 36, 745 (1992)

[6] Faulkner JDB, Anson JG, Tuite MF, Gene., 143, 13 (1994)

[7] Jia SR, Cui JD, Li Y, Biochem. Eng. J., 42, 193 (2008)

[8] Abdel-Fattah YR, Saeed HM, Gohar YM, Process Biochem., 40, 1707 (2005)

[9] Montgomery DC, Design and analysis of experiments, Singapore, Wiley Press, 125 (1984)

[10] Liyana-Pathirana C, Shahidi F, Food Chem., 9, 347 (2005)

[11] Lee H, Song M, Wang SH, Process Biochem., 38, 1685 (2003)

[12] Bocchini DA, Alves-Prado HF, Baida LC, Process Biochem., 38, 727 (2002)

[13] Majumader A, Goyal A, Bioresour. Technol., 99(9), 3685 (2008)

[14] Delisa MP, Rao G, Weigand WA, Biotech. Bioeng., 6, 554 (1999)

[15] LEE JH, YOO YJ, PAR KH, Korean J. Chem. Eng., 8(1), 39 (1991)

[16] Haghighi B, Shahidi D, Papari MM, Najafi M, Korean J. Chem. Eng., 24(1), 1 (2007)

[17] Sen R, J. Chem. Technol. Biotechnol., 68(3), 263 (1997)

[18] Akhnazarova S, Kefarov V, Experiment optimization in chemistry and chemical engineering, Moscow, Mir Publishers, 135 (1982)

[19] Khuri AI, Cornell JA, Response surfaces: design and analysis, New York, Marcel Dekker, 211 (1987)