The biomimetic phospholipid anti-biofouling multilayers were constructed on the biomedical poly(ethylene terephthalate) (PET) through the combination of layer-by-layer assembly and Michael addition reaction. Two biomacromolecules with opposite charges, alginate and chitosan, were sequentially adsorbed onto PET samples. The assembled multilayer was subsequently crosslinked with glutaraldehyde and biomimetic phospholipids was introduced into the assembled multilayer through the Michael addition of 2-methacryloyloxyethyl phosphorylcholine (MPC). The multilayer and phospholipid-modified PETs showed excellent hemocompatibility. (c) 2008 Elsevier B. V. All rights reserved.