To develop a microbial treatment of edible oil-contaminated wastewater, microorganisms capable of rapidly degrading edible oil were screened. The screening study yielded a yeast coculture comprising Rhodotorula pacifica strain ST3411 and Cryptococcus laurentii strain ST3412. The coculture was able to degrade efficiently even at low contents of nitrogen ([NH4-N] = 240 mg/L) and phosphorus sources ([PO4-P] = 90 mg/L). The 24-h degradation rate of 3,000 ppm mixed oils (salad oil/lard/beef tallow, 1:1 w/w) at 20A degrees C was 39.8% A +/- 9.9% (means +/- standard deviations of eight replicates). The highest degradation rate was observed at 20A degrees C and pH 8. In a scaled-up experiment, the salad oil was rapidly degraded by the coculture from 671 A +/- 52.0 to 143 A +/- 96.7 ppm in 24 h, and the degradation rate was 79.4% A +/- 13.8% (means +/- standard deviations of three replicates). In addition, a repetitive degradation was observed with the cell growth by only pH adjustment without addition of the cells.