A fed-batch culture system with constant feeding (glucose 80 g L-1, 0.25 ml min(-1)) was used to study the influence of glucose on cell dry weight and exopolysaccharides production from submerged Tremella fuciformis spores in a 5-L stirred-tank bioreactor. The results showed that high levels of cell mass (9.80 g L-1) and exopolysaccharides production (3.12 g L-1) in fed-batch fermentation were obtained after 1 h of feeding, where the specific growth rate (mu) and exopolysaccharides yield on substrate consumed (YP/S) were 0.267 d(-1) and 0.14 g g(-1). Unlike batch fermentation, maximal cell mass and exopolysaccharides production merely reached 7.11 and 2.08 g L-1; the specific growth rate (mu) and exopolysaccharides yield on substrate consumed (YP/S) were 0.194 d(-1) and 0.093 g g(-1), respectively. It is concluded that the synthesis of exopolysaccharides can be promoted effectively when feeding glucose at a late exponential phase.