The factors for selective production of N-alpha-benzyloxycarbonyl-L-aminoadipate-delta-semialdehyde (N alpha-Z-L-AASA) and N-alpha-benzyloxycarbonyl-L-aminoadipic acid (N-alpha-Z-L-AAA) from N-alpha-benzyloxycarbonyl-L-lysine (N-alpha-Z-L-lysine) by Rhodococcus sp. AIU Z-35-1 were analyzed. The cultivation time was important for selective production of N-alpha-Z-L-AASA, since N-alpha-Z-L-lysine oxidizing enzyme reached maximum at the early stage of cell growth and then decreased. The differences of enzyme activities of N-alpha-Z-L-lysine oxidizing enzyme and N-alpha-Z-L-AASA dehydrogenase in pH and temperature also affected the selective production of Nu-Z-L-AASA. For efficient production of Na-Z-L-AAA, it was important for cultivation time that N-alpha-Z-L-AASA dehydrogenase activity be higher than N-alpha-Z-L-lysine oxidizing enzyme activity, since a high concentration of N-alpha-Z-L-AASA inhibited N-alpha-Z-L-AASA dehydrogenase activity. The optimum pH of N-alpha-Z-L-AAA production was affected by the instability of N-alpha-Z-L-AASA dehydrogenase in the alkaline pH region.