We report on two-photon luminescence of silver nanoparticles grown on non-crystalline mesoporous TiO2 issued of the sol-gel process. The growth is achieved by surface photocatalytic reduction from silver nitrate solution. A structural hierarchy of this particular TiO2 system results in a characteristic distance between nanoparticles similar to 10-100 nm that is optimal for the electromagnetic field enhancement. The microscope images evidence multiple bright luminescence spots at two- photon near-IR femtosecond laser excitation due to the field enhancement effect. The spectra suggest homogeneous size distribution of emitting silver clusters over the sample area on the scale below 100 nm. (c) 2008 Elsevier B. V. All rights reserved.