Biotechnology Letters, Vol.30, No.3, 493-496, 2008
An efficient process for production and purification of hyaluronic acid from Streptococcus equi subsp zooepidemicus
Growth of Streptococcus zooepidemicus in a 10 l bioreactor with 50 g sucrose/l and 10 g casein hydrolysate/l gave 5-6 g hyaluronic acid/l after 24-28 h. Purification of hyaluronic acid gave a recovery of 65% with the final material having an Mr of similar to 4 x 10(6) Da with less than 0.1% protein.