Journal of the American Chemical Society, Vol.130, No.8, 2436-2437, 2008
Intramolecular acid-base catalysis of a phosphate diester: Modeling the ribonuclease mechanism
The hydrolysis of the phosphate diester bis(2-(1-methyl-1H-imidazolyl)pheny) phosphate BMIPP was evaluated as an intramolecular model for the ribonuclease A mechanism. Kinetic and thermodynamic data, isotope effects and ESI-MS and ESI-MS/MS analysis support the proposed intramolecular general acid-base catalysis by the imidazole groups.