A synthetic peptidolipid consisted of a hydrocarbon chain with a chain length of C18 and a peptide moiety of IIGLM terminated with an amine group, designated as C(18)IIGLM-NH2, has been employed as a biomimic model compound of amyloid peptide for exploring molecular interaction and orientation with the use of the Langmuir monolayer and Langmuir-Blodgett film techniques. Inspired by a well-known fact that a stain reagent, Congo red (CR), binds well to the amyloid-mimic part (IIGLM), inhibition of molecular aggregation of C(18)IIGLM-NH2 by interaction with CR was expected, and it has been investigated by use of surface pressure-area isotherm, surface dipole moment-area isotherm, Brewster-angle microscopy, and UV-vis/infrared spectroscopie. It has been revealed that monomeric CR molecules whose long axis is parallel to the Langmuir monolayer surface are penetrating the C(18)IIGLM-NH2 Langmuir monolayer, which plays a role of inhibition of molecular aggregation via hydrogen bonding.