The conditions for tricaprylin synthesis using Lipozyme (immobilized 1,3-specific Mucor miehei lipase) in a medium solely composed of glycerol and caprylic acid (octanoic acid) in stoichiometric amounts are 60 degrees C with air ventilation in an open batch. The kinetics of mono-, di-, and trioctanoylglycerol synthesis and caprylic acid esterification are correlated with those corresponding to water production and water activity alone the esterification reaction, The technical feasibility of studying the time course of the reaction and water loss monitoring only by weight and Karl-Fisher measurements is also described.