PVA-GA-CS/PVA-Fe-SA bipolar membrane was prepared by a paste method. PVA-sodium alginate (SA) and PVA-chitosan (CS) were crosslinked by FeCl3 and glutaraldehyde (GA), respectively. The charge densities of PVA-CS and PVA-SA solutions were determined by the colloid titration. The swelling level of bipolar membrane was in the range of 25-85%, meanwhile the permeability and the ion-exchange capacity as well as co-ion transport properties was investigated. FTIR was applied to analyze the functional groups of the bipolar membrane. Furthermore, SEM photographs of the BPM cross-section illustrated a structure that consists of an anion layer (PVA-GA-CS) and a cation layer (PVA-Fe-SA). TG analysis of PVA-GA-CS/PVA-Fe-SA bipolar membranes exhibited a good thermal stability. PVA-GA-CS/PVA-Fe-SA bipolar membranes were used as the separator in the electrolysis cell for electro-generation of 2,2-dimethyl-3-hydroxyprop ionic acid. The average current efficiency was 52.2%. and the highest current efficiency reached 68.9%. (C) 2007 Elsevier B.V. All rights reserved.