Investigation on Structure and Properties of a Novel Designed Peptide with Half-Sequence Ionic Complement

Li-Ping Ruan; Han-Lin Luo; Hang-Yu Zhang; Xiaojun Zhao

Macromolecular Research, Vol.17, No.8, 597-602, August, 2009

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Investigation on Structure and Properties of a Novel Designed Peptide with Half-Sequence Ionic Complement

Li-Ping Ruan, Han-Lin Luo, Hang-Yu Zhang, and Xiaojun Zhao^†

Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, No.1, Ke Yuan 4th Street, Gao Peng Road, Chengdu, 610041, Sichuan, PR China

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Although the existing design principle of full-sequence ionic complement is convenient for the development of peptides, it greatly constrains the exploration of peptides with other possible assembly mechanisms and different yet essential functions. Herein, a novel designed half-sequence ionic complementary peptide (referred to as P9), AC-Pro-Ser-Phe-Asn-Phe-Lys-Phe-Glu-Pro-NH2, is reported. When transferred from pure water to sodium chloride solution, P9 underwent a dramatic morphological transformation from globular aggregations to nanofibers. Moreover, the rheological experiment showed that the P9 could form a hydrogel with a storage modulus of about 30 Pa even at very low peptide concentration (0.5% (wt/vol)). The P9 hydrogel formed in salt solution could recover in a period of about 1,800 sec, which is faster than that in the pure water. The data suggested that the half-sequence, ionic complementary peptide might be worthy of further research for its special properties.

Keywords:peptide;half-sequence ionic complement;sodium chloride;conformation;fiber;hydrogel

[References]

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[1] Zhang S, Holmes T, Lockshin C, Rich A, Proc. Natl. Acad. Sci. USA, 90, 3334 (1993)

[2] Zhang S, Marini DM, Hwang W, Santoso S, Curr. Opin. Chem. Biol., 6, 865 (2002)

[3] Yokoi H, Kinoshita T, Zhang S, Proc. Natl. Acad. Sci. USA, 102, 8414 (2005)

[4] Zhang SG, Altman M, React. Funct. Polym., 41(1), 91 (1999)

[5] Zhang S, Gelain F, Zhao X, Semin. Cancer Biol., 15, 413 (2005)

[6] Zhang S, Mater. Today, 6, 20 (2003)

[7] Holmes TC, De Lacalle S, Su X, Liu G, Rich A, Zhang S, Proc. Natl. Acad. Sci. USA, 97, 6728 (2000)

[8] Zhao X, Nagai Y, Reeves PJ, Kiley P, Khorana HG, Zhang S, Proc. Natl. Acad. Sci. USA, 103, 17707 (2006)

[9] Aggeli A, Bell M, Boden N, Keen JN, Knowles PF, Mcleish TC, Pitkeathly M, Radford SE, Nature, 386(6622), 259 (1997)

[10] Caplan MR, Moore PN, Zhang SG, Kamm RD, Lauffenburger DA, Biomacromolecules, 1(4), 627 (2000)

[11] Caplan MR, Schwartzfarb EM, Zhang S, Kamm RD, Lauffenburger DA, Biomaterials, 23, 219 (2002)

[12] Collier JH, Hu BH, Ruberti JW, Zhang J, Shum P, Thompson DH, Messersmith PB, J. Am. Chem. Soc., 123(38), 9463 (2001)

[13] Ruan L, Zhang H, Luo H, Liu J, Tang F, Shi Y, Zhao X, Proc. Natl. Acad. Sci. USA, 106, 5105 (2009)

[14] Sreerama N, Woody RW, Anal. Biochem., 287, 252 (2000)

[15] Khang G, Rhee JM, Jeong JK, Lee JS, Kim MS, Cho SH, Lee HB, Macromol. Res., 11(4), 207 (2003)

[16] Nahar S, Tajmir-Riahi HA, J. Inorg. Biochem., 58, 223 (1995)

[17] Laussac JP, Orlewski P, Cung MT, Coord. Chem. Rev., 149, 179 (1996)

[18] Ferrari R, Bernes S, de Barbarin CR, Mendoza-Diaz G, Gasque L, Inorg. Chim. Acta, 339, 193 (2002)

[19] Benzakour M, McHarfi M, Cartier A, Daoudi A, J. Mol. Struct., 710, 169 (2004)

[20] Wu L, Meurer EC, Young B, Yang P, Eberlin MN, Cooks RG, Int. J. Mass Spectrom., 231, 103 (2004)

[21] Gates WD, Rostas J, Kakati B, Ngu-Schwemlein M, J. Mol. Struct., 733, 5 (2005)

[22] Ghosh S, Singh P, Verma S, Tetrahedron, 64, 1250 (2008)

[23] Chen P, Kim HS, Park CY, Kim HS, Chin IJ, Jin HJ, Macromol. Res., 16(6), 539 (2008)