Applied Chemistry, Vol.13, No.1, 17-20, April, 2009
N,O와 N-O Carboxymethyl Chitosan의 NMR 기능을 이용한 분광학적 특성분석
NMR spectroscopic characterization of N,O and N-O Carboxymethyl Chitosan
Carboxymethyl chitosan (CMCh) was prepared by chemical reaction with mono-chloroacetic acid and glyoxylic acid to low molecular weight water soluble chitosan (LMWSC) of C6-0H (O-Carboxymethyl chitosan) , C2-NH2 (N-CarboxymethyIc chitosan) and both C6-0H and C2-NH2 (N,O-Carboxymethyl chitosan) group to apply as an ionic -drug carrier. The structure were characterized by I H - NMR, 13C-NMR, DEPT 135 and HMQC (Heteronuclear Multiple Quantum
Correlation). Degree of substitution (DS) and degree of deacetylation (DDA) of CMCh was determined by 1H - NMR.